H2N2V1, Main, Exploration, bibRecord, 001042

Single-Reaction Genomic Amplification Accelerates Sequencing and Vaccine Production for Classical and Swine Origin Human Influenza A Viruses ▿

Identifieur interne : 001042 ( Main/Exploration ); précédent : 001041; suivant : 001043

Single-Reaction Genomic Amplification Accelerates Sequencing and Vaccine Production for Classical and Swine Origin Human Influenza A Viruses ▿

Auteurs : Bin Zhou ; Matthew E. Donnelly ; Derek T. Scholes ; Kirsten St. George ; Masato Hatta ; Yoshihiro Kawaoka [États-Unis] ; David E. Wentworth

Source :

Journal of Virology [ 0022-538X ] ; 2009.

RBID : PMC:2748056

Abstract

Pandemic influenza A viruses that emerge from animal reservoirs are inevitable. Therefore, rapid genomic analysis and creation of vaccines are vital. We developed a multisegment reverse transcription-PCR (M-RTPCR) approach that simultaneously amplifies eight genomic RNA segments, irrespective of virus subtype. M-RTPCR amplicons can be used for high-throughput sequencing and/or cloned into modified reverse-genetics plasmids via regions of sequence identity. We used these procedures to rescue a contemporary H3N2 virus and a swine origin H1N1 virus directly from human swab specimens. Together, M-RTPCR and the modified reverse-genetics plasmids that we designed streamline the creation of vaccine seed stocks (9 to 12 days).

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2748056

DOI: 10.1128/JVI.01109-09
PubMed: 19605485
PubMed Central: 2748056

Affiliations:

Le document en format XML

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Single-Reaction Genomic Amplification Accelerates Sequencing and Vaccine Production for Classical and Swine Origin Human Influenza A Viruses ▿

Single-Reaction Genomic Amplification Accelerates Sequencing and Vaccine Production for Classical and Swine Origin Human Influenza A Viruses ▿

Source :

Abstract

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